KIF2C antibody (AA 50-100)

Catalog No. ABIN7454675

This anti-KIF2C antibody is a Rabbit Polyclonal antibody detecting KIF2C in WB, IP and IHC (fp). Suitable for Human.

Quick Overview for KIF2C antibody (AA 50-100) (ABIN7454675)

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF2C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KIF2C Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-MCAK Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 50 and 100

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-MCAK antibody A300-807A or A300-808A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KIF2C

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Alternative Name: MCAK

Background: Background: Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. MCAK (Mitotic Centromere-Associated Kinesin) is the founding member of kinesin-13 proteins. There are 3 independent genes coding for kinesin-13 proteins kif2a, kif2b, and kif2c (MCAK). Instead of functioning as a translocator of microtubules MCAK is a depolymerizer that is primarily responsible for releasing improper microtubule-kinetochore attachments during cell division.

Gene ID: 11004

NCBI Accession: NP_006836

UniProt: Q99661

Pathways: Microtubule Dynamics